| Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element S Chong, FB Mersha, DG Comb, ME Scott, D Landry, LM Vence, ... Gene 192 (2), 271-281, 1997 | 925 | 1997 |
| Functionally diverse type V CRISPR-Cas systems WX Yan, P Hunnewell, LE Alfonse, JM Carte, E Keston-Smith, ... Science 363 (6422), 88-91, 2019 | 494 | 2019 |
| Cas13d is a compact RNA-targeting type VI CRISPR effector positively modulated by a WYL-domain-containing accessory protein WX Yan, S Chong, H Zhang, KS Makarova, EV Koonin, DR Cheng, ... Molecular cell 70 (2), 327-339. e5, 2018 | 469 | 2018 |
| Protein splicing involving the Saccharomyces cerevisiae VMA intein: the steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in … S Chong, Y Shao, H Paulus, J Benner, FB Perler, MQ Xu Journal of Biological Chemistry 271 (36), 22159-22168, 1996 | 374 | 1996 |
| Utilizing the C-terminal cleavage activity of a protein splicing element to purify recombinant proteins in a single chromatographic step S Chong, GE Montello, A Zhang, EJ Cantor, W Liao, MQ Xu, J Benner Nucleic acids research 26 (22), 5109-5115, 1998 | 328 | 1998 |
| Characterization of a self-splicing mini-intein and its conversion into autocatalytic N-and C-terminal cleavage elements: facile production of protein building blocks for … S Mathys, TC Evans Jr, IC Chute, H Wu, S Chong, J Benner, XQ Liu, ... Gene 231 (1-2), 1-13, 1999 | 295 | 1999 |
| Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein S Chong, KS Williams, C Wotkowicz, MQ Xu Journal of Biological Chemistry 273 (17), 10567-10577, 1998 | 236 | 1998 |
| Protein splicing of the Saccharomyces cerevisiae VMA intein without the endonuclease motifs S Chong, MQ Xu Journal of Biological Chemistry 272 (25), 15587-15590, 1997 | 176 | 1997 |
| Modified proteins comprising controllable intervening protein sequences or their elements methods of producing same and methods for purification of a target protein comprised … DG Comb, FB Perler, WE Jack, MQ Xu, RA Hodges, CJ Noren, ... US Patent 5,834,247, 1998 | 161 | 1998 |
| Overview of cell‐free protein synthesis: historic landmarks, commercial systems, and expanding applications S Chong Current protocols in molecular biology 108 (1), 16.30. 1-16.30. 11, 2014 | 137 | 2014 |
| [24] Fusions to self-splicing inteins for protein purification MQ Xu, H Paulus, S Chong Methods in Enzymology 326, 376-418, 2000 | 115 | 2000 |
| A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based JR Hillebrecht, S Chong BMC biotechnology 8, 1-9, 2008 | 83 | 2008 |
| Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli SS Sharma, S Chong, SW Harcum Journal of biotechnology 125 (1), 48-56, 2006 | 75 | 2006 |
| In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme H Asahara, S Chong Nucleic acids research 38 (13), e141-e141, 2010 | 69 | 2010 |
| Reconstitution of translation from Thermus thermophilus reveals a minimal set of components sufficient for protein synthesis at high temperatures and functional … Y Zhou, H Asahara, EA Gaucher, S Chong Nucleic acids research 40 (16), 7932-7945, 2012 | 67 | 2012 |
| Visualization of coupled protein folding and binding in bacteria and purification of the heterodimeric complex H Wang, S Chong Proceedings of the National Academy of Sciences 100 (2), 478-483, 2003 | 66 | 2003 |
| Protein synthesis using a reconstituted cell‐free system C Tuckey, H Asahara, Y Zhou, S Chong Current protocols in molecular biology 108 (1), 16.31. 1-16.31. 22, 2014 | 64 | 2014 |
| Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins A Zhang, SM Gonzalez, EJ Cantor, S Chong Gene 275 (2), 241-252, 2001 | 59 | 2001 |
| Intein-mediated protein ligation of expressed proteins TC Evans, MQ Xu, S Chong US Patent 6,849,428, 2005 | 57 | 2005 |
| Intein-mediated rapid purification of Cre recombinase EJ Cantor, S Chong Protein Expression and Purification 22 (1), 135-140, 2001 | 56 | 2001 |
